Ctd110.6 antibody

Author: aodw

August undefined, 2024

WebDec 9, 2016 · The RL2 epitope was detected on Sp1 during 2DG treatment, and the level was transiently increased at 24 h. In contrast, the CTD110.6 epitope became detectable on Sp1 over 72 h after 2DG treatment, and then the other proteins containing CTD110.6 epitopes also appeared in the cell lysates and the anti-Sp1 antibody precipitates. WebIn 2008, the Okajima group originally discovered the O-GlcNAc modification on Drosophila Notch EGF20 by detecting O-glycans on the ECD of Drosophila Notch using an antibody that recognizes O-GlcNAc (CTD110.6) . Later, O-GlcNAc modifications of the EGF repeats in Drosophila Delta, Serrate, and Dumpy were subsequently discovered [86,87].

Goat anti-Mouse IgM (Heavy chain) Cross-Adsorbed Secondary Antibody …

WebThe antibody CTD110.6 is often used to detect changes in the O-GlcNAc modification. Recently, it has been demonstrated that CTD110.6 recognizes N-linked N,N'-diacetylchitobiose, which is thought ... WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc … phosphatlösung formel

Anti-O-GlcNAc Antibody, clone CTD110.6 MABS1254 - EMD Mi…

WebMonoclonal Antibody for studying Ser/Thr O-GlcNAc. Cited in 70 publications. Validated for Western Blotting. Highly specific and … WebCTD110.6, 18B10 and 9D1 are anti-O-GlcNAc monoclonal antibodies. B. HEK 293T cells were treated with DMSO, 50 μM TG or 50 μM 5SG for 24 hours, 200 nM rapamycin … WebApr 18, 2014 · Binding by antibodies CTD110.6, #3, and #10 was inhibited by 5 m m GlcNAc, but not 5 m m GalNAc, and was thus specific for recognition of O-GlcNAc. However, binding of antibodies 10D8, RL2, and #14 was not inhibited by the presence of 5 m m GlcNAc. Therefore, only antibodies CTD110.6, #3, and #10 should be used for cell … how does a stampede happen

Anti O Glcnac Ctd110 6 Santa Cruz Biotechnology Bioz

O-GlcNAc Monoclonal Antibody (CTD110.6) (61453)

WebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … WebApr 19, 2011 · Results. When T24 human bladder cancer cells were subjected to glucose deprivation, the expression of proteins detected by the O-GlcNAc specific antibody … phosphatizing stainless steelWebSimilar results were obtained for wild-type HEK293T cells (not shown). (C) Expression and O-GlcNAcylation of Notch1, Notch1Δ2xO-GlcNAc and Notch1Δ4xO-GlcNAc were analyzed by immunoprecipitation (IP) using NOTCH1 (8G10) antibody, followed by immunoblotting with CTD110.6 O-GlcNAc or NOTCH1 antibodies. phosphatizing pressure washer

"WebThus, with careful controls, antibodies CTD110.6 (IgM), #3 (IgG), and #10 (IgG) can be used to detect membrane and secreted proteins modified by O-GlcNAc on EGF repeats. Secreted and cell surface glycoproteins carry a wide spec-trum of N-and O-glycans that are synthesized during transit " - Ctd110.6 antibody

Ctd110.6 antibody

O-GlcNAc (O-linked N-acetylglucosamine) antibody - Active Motif

WebFormulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA. Preparation. The antibody was purified by chromatography and conjugated with … WebMay 18, 2024 · 3. Incubate the blots with CTD110.6 (diluted to 1 μg/ml in antibody dilution buffer), in duplicate, with and without 100 mM GlcNAc, overnight at 4°C. An antibody that has been antigen purified using a GlcNAc-agarose column yields higher-quality blots. Commercially available CTD110.6 is purified by ammonium sulfate precipitation.

Did you know?

WebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and … WebO-GlcNAc (CTD110.6) is a mouse monoclonal antibody raised against a peptide containing serine-O-linked N-acetylglucosamine. PRODUCT Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of

WebO-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation. PLoS One. 2011 04 19;6(4):e18959. Zachara, Natasha E; Molina, Henrik; Wong, Ker Yi; Pandey, Akhilesh; Hart, Gerald W. The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA … WebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t …

WebO-GlcNAc Antibody (CTD110.6) is an IgM κ mouse monoclonal O-GlcNAc antibody (also designated O-linked β-N-acetylglucosamine antibody, O-β-GlcNAc antibody, O-linked … WebAnti-O-GlcNAc Antibody, clone CTD110.6 clone CTD110.6, from mouse; Synonyms: O-GlcNAc, beta-O-GlcNAc, O-Linked N-Acetylglucosamine, beta-O-linked N …

WebImmunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at …

WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O-GlcNAc (CTD110.6) antibody Invented at ... how does a standard automotive relay workWebTime course studies by western blot with the CTD110.6 antibody demonstrated that i.v. injection in rat at 50 mg/kg increased global protein O-GlcNAcylation in total brain homogenates, starting at 1 h and peaking at 10 h and decreased pS396 tau/Tau-5 starting at 1 h, peaking at 4 h, and restoring to normal at 16 h (Yuzwa et al., 2008). phosphatmangel ambossWebSpecificity. Clone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The … how does a stall converter workWebJul 23, 2024 · O-GlcNAc Antibody (CTD110.6) is a mouse monoclonal IgM κ O-GlcNAc antibody, cited in 62 publications, provided at 200 µg/ml; raised against O-GlcNAc … how does a stannah stairlift workWebOct 11, 2013 · For immunodetection, CTD110.6 antibody was used because it is widely used for the detection of O-GlcNAcylated proteins. Compared to the α-DG-Fc isolated from the control transfectants, immunoreactivity of α-DG-Fc towards CTD110.6 was elevated upon GTDC2 overexpression (Fig. 1A). how does a standing wave formWebTarget Information. CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b … how does a standby generator work for a homeWebMar 5, 2024 · Compared to immunostaining of O-GlcNAcylated proteins by CTD110.6 antibody, OGT immunostaining appears to be more cytosolic with less intense immunoreactivity in hippocampal CA1 (ratios of immunoreactivity intensity between CA1 to adjacent region are ~ 0.73 for OGT and ~ 2.5 for CTD110.6, respectively) or dentate … how does a standby generator work