WebA mixture of the positive and negative beads in the ArC Bead Kit (A 10346) were stained with LIVE/DEAD Fixable Violet Dead Cell Stain Kit (V34955), washed, and analyzed using a flow cytometry equipped with a 405/7nm … WebThese beads are mixed with inert, non-antibody binding beads. This allows researchers to generate artificial positive and negative fluorescence populations that mimic the heterogeneous populations of cells in a sample that are negative or positive for a given marker. ... BioLegend Compensation Beads stained with 23 different FITC-conjugated ...
Fluorescence Quantitation Bangs Laboratories, Inc.
WebBeads are suitable for labeling with mouse mAbs conjugated with violet fluorochromes, and for use as a compensation or general reference standard for detectors off of the violet laser. ... The FITC / PE Compensation Standard includes four microsphere populations that are ~7-9µm in diameter: an Autofluor™ population, and single populations ... WebDescription. Each BD Quantibrite™ PE tube contains a lyophilized pellet of beads conjugated with four levels of phycoerythrin (PE). The pellet is restrained in the bottom of the tube by a stainless steel retainer. The number of PE molecules per bead at each level varies among lots; lot-specific information is included with each kit. popla contact number uk
Phagocytosis Assay Kit (IgG FITC) - Cayman Chem
WebAmong those products you’ll find beads with these variations: • Ten fluorescent colors • Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm • Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine WebThe Setup Beads 3: Raw Beads are used to set up the PMT voltages of the classification channel APC (or AF647) and reporter channel PE. The Setup Beads 1: FITC Beads is not needed for this setup because no compensation is required if the setup procedure is closely followed. The Setup Beads 2: PE beads is not required, but can be used as a WebStep 1: It is necessary for compensation beads to bind the antibody or reagent used in the specific experiment. Use the selection guide to pick the correct compensation bead that binds the species or reagent and can be … popla grounds for appeal