Phleomycin plates
Webb1 feb. 2002 · Selection on phleomycin plates gave rise to resistant fungal colonies only when transformed A. tumefaciens was used for cocultivation together with AS, indicating that induction of the vir-genes was essential for T-DNA transfer between A. tumefaciens and hyphae of ecto- mycorrhizal fungi. WebbChemical structure of phleomycin D1. Phleomycins are a group of glycopeptide antibiotics found in Streptomyces which are closely related to bleomycin. [1] Examples include: …
Phleomycin plates
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Webb19 sep. 2013 · The pretreated MA plates were prepared by adding either 50 or 100 µg of Phleomycin (from a 20mg/mL stock) drop wise to each plate in three different regions. After allowing the antibiotic to dry (c.a., 30 min), the individual strains were plated across an entire plate. Resistance was evaluated after two days of growth at either 25°C or 13°C. Webb26 apr. 2024 · Finally, the colonies were tested for sensitivity towards phleomycin by transferring them using sterile toothpicks to YE-phleomycin plates. If colonies were unable to grow on these plates, it was an indication that the yeast cells eliminated the pUT332 plasmid during the non-selective prolonged growth period.
Webb产品描述. 腐草霉素（Phleomycin，CAS NO.：11006-33-0）是一种结构上类似博莱霉素（bleomycin）的糖肽类抗生素，分离自轮枝链霉菌（Streptomyces verticillus）。. 作用机理在于通过结合和插入DNA进而破坏双螺旋的完整性，有效作用大多数细菌、丝状真菌、酵母菌、植物和 ... WebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ...
WebbPhleomycin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS ... °C and number of cells were normalized to … Webb25 okt. 2013 · Most commercial preparations of phleomycin suggest using 10 μg/mL for selection of S. cerevisiae on YPD, as determined by Gatignol et al. . However, we …
Webb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, read and cite all the ...
WebbPhleomycin is a glycopeptide antibiotic and is a member of the bleomycin family. Application Phleomycin from Streptomyces verticillus has been used as an antibiotic in … how to set desktop notification for gmailWebbFig.2 Phleomycin test plates (1-3: 0, 25, 50 ug/mL) As the images shown above, the growing situation of wild type strain M. anisopliae on hygromycin or phleomycin plates … how to set development objectivesWebb3 apr. 2024 · Plates were incubated at 30°C for 4 days before photography. In summary, we demonstrated here that Zeocin, a phleomycin analog, can be used for selection of bleM … how to set details as default for windows 10WebbPhleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticillus . It binds and intercalates DNA thus destroying the … note cards template wordWebbPhleomycin-resistant transformants are selected with 10-50 µg/ml of phleomycin in the regeneration medium, depending on the sensitivity of the host strain. Selectivity can be … note cards studyingWebb1 jan. 2000 · It was determined that no detectable growth occurred on plates containing 200 μg of G418 per ml or on plates containing 250 μg of phleomycin per ml at pH 7.0. … note cards research paperWebb29 apr. 2024 · Given phleomycin causes global DSBs by an unknown mechanism, we also tested the effects of deletion of DMA1 and DMA2 on plates containing an established … how to set dewenwils timer